Green Synthesis of Silver Nanoparticles Using Piper betle Leaf Extracts and Its Antimicrobial Properties

Nooramalina Azhar, Khairul Farihan Kasim, Akmal Hadi Ma'Radzi


Objective - This project was aimed to evaluate the synthesized silver nanoparticles from Piper betle and its antimicrobial properties.

Methodology/Technique - Silver nanoparticle was synthesized by adding P. bettle leaves extract with silver nitrate concentration (varies the concentration and volume of silver nitrate). The formation of the silver nanoparticles was observed by the colour changes from yellowish to brown and confirmed with UV-vis spectrum at 452 nm. Antimicrobial activity was evaluated by using agar well plate against Escherichia coli (gram-negative bacteria), Pseudomonas aeruginasa (gram-positive bacteria) and the Aspergillus niger (fungi). The characterization of the size and shape of the synthesized silver nanoparticles were analysed using Field Emission Scanning Electron Microscope (FESEM) and elemental composition of the samples was done using Energy-dispersive X-ray spectroscopy (EDX).

Findings - The results show that synthesized silver nanoparticles of P. betle at concentration of silver nitrate at 1 mM with ratio of extract to silver nitrate volume is 1:9 exhibit admirable zone of inhibition against E. coli, P. aeruginosa and A. Niger (16.5±0.29, 14.3±0.21 and 14.8±0.17 mm, respectively). Field emission scanning electron microscope (FESEM) was revealed that synthesized silver nanoparticles were in spherical shape; ranged from 15-19 nm in size. The presence of silver element was observed by using energy-dispersive spectroscopy (EDX), which suggests that silver is presence as the primary element with a strong absorption at 2.8 keV.

Novelty - P. betle leaf is a promising antimicrobial agent against the plant pathogens. The incorporation between silver nanoparticle and this plant will clearly demonstrate the newly synthesized of silver nanoparticles. Hence, the green synthesized of silver nanoparticles is one of the good source, which is easily produced and extensively useful in biomedical and agricultural applications

Type of Paper: Empirical

Keywords: Antimicrobial; aqueous extract; Piper betle; silver nanoparticles.


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